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Genes essential for the killing phase of apoptosis, whose mutation completely eliminates apoptosis, were devoid of the small DNA pieces (Figure 2A).
Viability of exponentially grown wild-type E. coli MG1655 treated with TMP at 50 μg/ml could be described by a typical by-phasic curve: a prolonged (120 min) lag followed by an exponential killing phase, with 17% of initial colony forming units (CFU) remaining after 3 hours of treatment.
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A characteristic feature of this type of resistance is a biphasic antibiotic killing curve, in which a fast initial kill phase is followed by much slower killing.
Fluoroquinolones are widely advocated as excellent first choice drugs for Salmonella PJIs given their high oral bio-availability and ability to penetrate biofilms and kill stationary phase as well as active phase bacteria.
The potency of HT61 was significantly higher than that which has been reported for the anti-stationary phase agent daptomycin [20] that required 50 µg/ml to completely kill stationary phase MSSA and MRSA (Fig. 2).
High glucose also activates DNA damage responses and preferentially kills stationary phase cells that fail to arrest growth in G0/G1.
The computer simulation allowed us to discard any data not including all the effects of block and killing in each phase reported below.
In fact if the drug kills in one phase and blocks in the same phase the percentage of cells in that phase might be similar to that of untreated control cells.
Materials and methods In a first set of experiments, we explored PMN functions (ROS production, NETosis and bacteria killing ability) during late phase of sepsis in a murine model of CLP-induced sepsis.
As noted, potent replicating-active compounds may register as potential nonreplicating-active compounds by killing in the outgrowth phase of the assay.
During the first half of the 20th century, this relief came in the form of barbiturates, which, due to the fact that they were both addictive and very good at killing people, were eventually phased out of the market.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com