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As observed in Fig. 1a, significant amounts of Mn2+ were produced in bacterial treatments, which was considerably higher than the concentrations of Mn2+ in sterile control and killed control.
Considering the results of killed control and the positive correlation between the Mn2+ release rates and protein concentrations, we speculate the bioreduction process may be an enzymatic reaction, which needs further demonstration.
To their surprise, the scientists found that when they added tPA to their culture medium, brain cells were completely protected against the zinc that killed control cells.
Mice immunized with r-αCS were protected against intramuscular challenge with 5 × LD100 doses of C. perfringens and S. aureus alpha toxins with >80% survival, which killed control animals within 48 72 h.
Abe's body is immobile and vulnerable whilst possessing someone else, whereas if his host is killed, control will return to Abe's body.
One tube was amended with 90 µl of 100% ice-cold trichloroacetic acid (TCA) as a killed control.
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Values for killed controls were subtracted from those for live samples.
In the maximal killing control wells, cells were lysed by addition of 1/10 volume of 9% Triton X-100 45 min before crystal violet staining.
Killed controls had the TCA added prior to the addition of the sample and their values were subtracted from those of the experimental samples.
The "armies of business" fought and killed to control the mines and send it to us.
The government began two pilot badger culls in Gloucestershire and Somerset last year to assess whether enough badgers could be killed to control the spread of TB to cattle.
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