In what could turn out to be a groundbreaking ruling, the panel said that Arizona could not go ahead with the execution unless it revealed details about the combination of two medical drugs with which it intended to kill Wood on the grounds that the prisoner had a first amendment right to access to government proceedings.
Many of the interior materials were selected both for their beauty as well as their reflection of ongoing faculty and student research of sustainable and innovative construction materials, including Pine Beetle Kill Wood and Cross Laminated Timber (CLT) Panels.
Representative composite images of control and poly(I) treated cells after phagocytosis of unopsonized latex beads, commercial killed Wood strain, and live Wood strain are shown in figure 5, and are consistent with the automated analysis results.
Protein kinase inhibition by staurosporine inhibited uptake of live or commercial killed Wood strain, and PKC inhibition by Gö 6976 inhibited uptake of commercial killed Wood strain only.
Our results using this killed Wood strain in the experiments reported here are quite similar to prior findings.
Many of these studies, including our own efforts, have utilized a commercial preparation of killed Wood strain bacteria [2], [4], [6], [8], [9].
At 10 µg/ml, poly(I) reduced binding of commercial killed Wood strain by 82% compared with control (p<.01) and had no effect on binding of other S. aureus forms (figure 4D).
GM-MФs in suspension were treated with 10 µg/ml poly(I) or chondroitin sulfate, and incubated with S. aureus (commercial killed Wood or RN6390 strain) or green fluorescent latex beads and analyzed by flow cytometry.
Staurosporine blocked internalization of unopsonized beads by 59% (p<.001), commercial S. aureus killed Wood strain by 18% (p<.01) and live Wood strain by 29% (p<.001), but had no significant effect on internalization of other unopsonized strains of S. aureus or IgG-opsonized RN6390 (figure 7C).
At 100 µg/ml poly(I), binding of commercial killed Wood strain was inhibited by 80% (p<.01), binding of RN6390 strain was partially inhibited (similar to our findings with the human macrophages, ∼45%, p<.05) and binding of live Wood and Cowan 1 strains was unaffected (figure 4D).
These results suggest that SRs do not play a major role in the unopsonized phagocytosis of live or clinically relevant strains of S. aureus, and that the commercial killed Wood strain may be peculiar among S. aureus forms in its SR-mediated uptake.
