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The kill group was incubated for 30 min following treatment with serum-free media containing 100 μg/mL gentamicin.
A third set of samples (kill group) was treated with serum-free media containing 100 μg/mL gentamicin (Invitrogen) and incubated for 1 h to allow bacterial killing to occur in the RAW264.7 cells.
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This is the third time this year that a group of women suspected of being prostitutes has been killed; this group was the largest to date, according to morgue workers.
The cell killing functional group was higher in the co-upregulated unigenes, less expressed in the group downregulated unigenes by SAV-3 infection only and absent in the co-downregulated unigenes.
Six truck drivers were killed after their group was ambushed in 2004.
One animal from each group was killed every 15 days, unless they needed to be killed due to deteriorating health to examine Intracranial (i.c).
One goat from each group was killed at day 7 pi and the remaining goats were killed at day 14 pi.
Abu Musab al-Zarqawi, the former leader of the group, was killed in the province in 2006 by American bombs.
The leader of Colombia's largest guerrilla group was killed Friday in a military raid, Colombian government officials said.
The control group was killed after 24 h.
One of the group was killed when he struck a tree - but the others survived.
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