The laminin-rich Matrigel reconstituted basement membrane matrix is a key substrate for differentiation of mammary epithelial cells into spherical duct-like structures in culture.
GLN is a key substrate for the hexosamine biosynthetic pathway (HBP), which has been shown to induce HSP70.
Growing evidence has shown that transcription factors and co-factors are key substrates for SUMOylation5.
For the latter, TAp73 and its interacting regulator YAP, have been demonstrated to be key substrates for c-Abl.
This metabolic lesion may compromise the availability of GC-derived pyruvate, the key energy substrate for oocyte development and maturation, and so negatively impact on oocyte quality.
Diaminobenzidine (Sigma) in phosphate-buffered saline was used as substrate for development of the peroxidase reaction.
In addition, molecules identified as crucial for development in vivo may likely serve as key substrate molecules for potential small molecule drug target intervention and for the establishment of conditions for stem cell manipulation such as in vitro organ formation.
Oxygen (O2) is the key substrate in metabolism and essential for aerobic organisms to produce energy.
After three washes, streptavidin-ALP (Mabtech, Nacka Strand, Sweden) was added for 1 h at RT, followed by the addition of the substrate for spot development (Mabtech).
3-Amino-9-ethyl carbazol (AEC) was used as the substrate for color development.
