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GABA signaling is also important in AGRP neurons, and synaptic vesicle glycoprotein 2C (Sv2c, +5.4-fold, q = 5e−10), which regulates the readily releasable pool (Xu and Bajjalieh, 2001) is increased, as is Snap25 (+2.1-fold, p = 1.5e−6), a key SNARE complex component responsible for vesicle fusion.
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Thus, by facilitating the uptake, secretion and recycling of key biological molecules, SNARE proteins maintain cellular inventory and facilitate various dynamic cellular processes described above.
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Key proteins regulating SNARE-mediated fusion at the synapse are the calcium sensor synaptotagmin and complexin (CPX) (Brose et al., 1992; McMahon et al., 1995; Fernandez-Chacon et al., 2001; Giraudo et al., 2006; Sudhof and Rothman, 2009).
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It is well known that SAR/ARF GTPases regulate budding processes, and RAB GTPases and soluble N-ethyl-maleimide sensitive factor attachment protein receptors (SNAREs) are key regulators of membrane attaching and fusion.
Given the Melville connection (the author has even snared a key role in the film) and the fact that Charles Leavitt's script was based on the award-winning nonfiction book by Nathaniel Philbrick, it's a bit of a surprise that the dramatic elements don't weigh more heavily in the equation here.
Soluble N-ethylmaleimide-sensitive factor (NSF) adaptor proteins (SNAPs) receptors (SNAREs) are key components of the intracellular vesicle-mediated transports that take place in eukaryotic cells and are distinguished by the presence of a common motif (SNARE motif).
SNARE proteins, and key regulators like synaptotagmin and complexin, can be ectopically expressed on the cell surface.
Regulating the microsecond to millisecond time scale dynamics in the neuronal SNAREs is likely to be a key feature that regulates assembly of the SNAREs and neurotransmitter release.
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