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In order to evaluate the molecular determinants that contribute to the reaction, the crystal structure of AVR4 was compared with those of avidin, streptavidin and key mutants of the two proteins in complex with biotinyl p-nitroanilide (BNA), the inert amide analogue of BNP.
In conclusion, to investigate fruit maturation and fruit softening we tilled a list of candidate genes and isolated key mutants that enhanced melon fruit quality (data not shown).
Similarly, LXB-injection did not lead to a decreased induction of Defensin, a gene that appears to be controlled by the imd pathway as observed here in key mutants (Fig. 1G).
Furthermore, the measurements herein show that key mutants retain WT-like activity, arguing against differential aggregation.
The representative traces from a few key mutants are shown in Supplemental Figure 3, Supporting Information.
In summary, the complete photocycles of avGFP and two key mutants have been measured with IR spectral resolution.
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Next generation NNRTIs are sought which possess both broad spectrum antiviral activity against key mutant strains and a high genetic barrier to the selection of new mutant viral strains.
We have designed and synthesized a series of novel pyrazole derivatives as a surrogate for pyrazolopyridine motif that were potent inhibitors of HIV-1 RT with nanomolar intrinsic activity on the WT and key mutant enzymes and potent antiviral activity in infected cells.
LXB-preinjection did not impair Drosomycin induction in wild-type or imd pathway (key) mutant flies (Fig. 1 D-F).
STS dif-key flies did not show an improved survival pattern compared to AL dif-key mutants (Fig 3A).
However, we found that Set2 levels were still decreased in a number of key checkpoint mutants tested (Supplementary Fig. 4e).
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