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Cells were selected using RPMI1640/DMEM F12(1:1) media supplemented with 2,5 ug/ml Puromycin for 5 days and kept under selective pressure.
The vtx2-phages characterising EAHEC seem to belong to a sub-population of vtx-phages kept under selective pressure and characterised by the presence of a gene encoding a tail fiber, which could be involved in the mechanism used to recognize the EAggEC.
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Although cell clones with homogenous transgene expression can be kept under selective conditions over a long period, this desirable phenotype can be progressively lost upon withdrawal of selective pressure [ 33].
MVP is under selective pressure to maintain structure and appropriate residues for docking.
If the apparently fast-evolving sites were under selective pressure, the saturation hypothesis would not hold.
Several genes involved in determining skin color have been under selective pressure in Europeans and East Asians.
And that, Dr. Wrangham said, suggests the males were no longer under selective pressure to get bigger themselves.
By contrast, the versions of the gene possessed by monkeys, dogs, cats and cows show no particular sign of being under selective pressure.
The genes under selective pressure found in one continent-based population or race are mostly different from those that occur in the others.
Transduced cell populations were than cultivated under selective pressure.
Interestingly, the orthologs in both the nematode and the fruitfly are still under selective pressure.
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