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After casting, the specimens were wrapped using a plastic sheet and kept under room temperature.
Twenty microliter of 85% hydrazine hydrate was dissolved in 60 mL of water, and then 6 mL CS2 was added dropwise, the reaction mixture was kept under room temperature for 1 h and then heated to 90°C for an additional 8 h.
Control plates were kept under room temperature in darkness.
The petri dishes were kept under room temperature for about 10 minutes.
However, under stress, oxygen demand likely is increased, thus possibly leading to intracellular hypoxia even in cells kept under room air.
Colour change was observed even in samples kept under room temperature from white > dark purple > purple and can be followed with the changes observed in the absorption spectra in the visible region.
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In our experiments, 50 ml of MB and 20 mg of catalysts were kept under dark room to get adsorption desorption equilibrium.
The system was shaken for 1 min and later kept under storage at room temperature (20 25 °C) for 24 h.
Then, the solution was added to 100 g of the oil and the reaction system was kept under stirring and room temperature for 1 h.
The set-up is then kept under stirring at room temperature with continuous hydrogen flush until a pH between 7 and 10 is attained.
Briefly, 10 mg HS-PEG-NH2 was mixed with 10 mg GNS in water and was kept under shake at room temperature for 2 h.
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