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For experimental tumor models, in-house SCID mice of 7 weeks of age were used, and the animals were kept under aseptic conditions until biodistribution was performed.
Mice were kept under aseptic conditions.
The fermentation, kept under aseptic conditions, was carried out by incubating the kombucha culture at 28 ± 1°C for 12 days.
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He was kept under review.
Cells were kept under normoxia.
Keep under the lights.
Keep under cover.
The environment was kept aseptic to ensure that the seedlings were disease- and contaminant-free before transplantation into pots.
In order to evaluate GmGER gene response to IAA in soybean development, 'Maple arrow' seedlings were surface sterilized and then placed on the soil under aseptic condition, and kept in growth chamber under white fluorescent light (600 umol m-2s-1, 16 h light/8 h dark) at 25°C and 90% relative humidity.
After 4 days of transfer to fresh media, the substrate 1 (600 mg) was dissolved in DMSO (10 mL) and evenly distributed among 10 culture flasks (1 liter) having 400 mL cell suspension media under aseptic conditions, which were kept for 15 days under the same conditions.
Control xenogeneic, untreated osteochondral grafts were harvested immediately prior to transplantation from fresh bovine shoulder joints under aseptic conditions, washed and kept in saline for only a few minutes before use.
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