And we'll keep supplementing Science special issues with career-related content.
The initial data analysis of the participants' transcripts was inductive, and no categories were specified in advance [ 9, 10] A code book was kept to supplement the discussion generated from reading the transcripts.
In addition, each subject kept a supplement diary and research assistants reviewed supplement diaries at each prenatal visit and collected data on side effects, dietary intake of DHA-rich foods, and adverse events including vaginal infections, vaginal bleeding, and preterm labor occurring during pregnancy.
The total DNA concentration (1 µg) was kept constant by supplementing with empty vector.
The total amount of nucleotide was kept constant by supplementing with pcDNA3.
The amount of DNA in the transfections was kept constant by supplementing with pCI plasmid DNA (Promega, WI, US).
In all experiments where multiple plasmids were used, the total plasmid amount was kept constant by supplementing with the empty vector.
TF1 human erythroid cells (American Type Culture Collection (ATCC)) were cultured in RPMI 1640 medium supplemented with 10% FBS and GM-CSF (2 ng ml−1) and 1% penicillin/streptomycin. HEK293T (ATCC) and Hela cells (ATCC) were kept in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin.
As a result, many of the building's original features the clay brick walls, the decorative wooden interiors and that timber frame were kept intact, albeit supplemented by tasteful modern touches.
Cells were kept in DMEM supplemented with 10% heat-inactivated fetal calf serum, 2 mM Glutamine, 50 U/ml penicillin and 50 µg/ml streptomycin [28].
SH-SY5Y neuroblastoma cells (ATCC) were kept in DMEM∶F12 (1∶1) supplemented with 2 mM glutamine, 1% non essential amino acids and 15% fetal bovine serum (FBS).
