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Extracts were kept ice-cold at all times.
Blood samples were kept ice-cold for a maximum of 30 minutes before RNA extraction.
Embryo Dissociation Medium (EDM) was freshly prepared using Leibovitz's L-15 Medium Life Technologiess, 11415 064) containing 10% FBS (Sigma, F-2442), and kept ice-cold prior to use.
Then the beaker was kept in ice cold water bath after addition of 100 ml de-ionized water in to it and allowed to precipitate PbSO4.
After harvesting the leaves were kept in ice cold water in either light or darkness for 20 minutes before the preparations were started.
The brains were quickly removed from the skulls and kept in ice cold culture dish for dissection.
Following surgery the tissues are kept in ice cold transportation medium (Eurocollins) lacking growth factors until cultivation.
Until it was connected to the aortic cannula on a standard Langendorff retrograde perfusion system, the hearts were kept on ice cold Hepes solution.
But these days refrigeration systems are needed to keep the ice cold at hockey arenas.
Keep the ice cold.
Keep ice in the tube along with the soda bottle so it stays ice-cold.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com