His imprisonment (1956 62) kept him dissociated from those errors of military conduct committed by the FLN, and, when he was freed after the Évian agreements with France were signed in 1962, his reputation was intact.
At 15 20 passages from the shift to the specific oxygen condition (1%, 2.5%, 5%, 20% O2), IhNSC were dissociated and kept in growing conditions for 24, 48 or 72 hours.
After 6/7 days of culture, neurospheres were collected by centrifugation, dissociated and kept in culture for additional 7 days by re-suspension in fresh DMEM-F12/Glutamax medium with growth factors (passage).
For lentiviral vector transduction, dissociated neural stem cells were kept in the same media at a 106 cells/ml density and incubated for 2 hours with 50 ng/p24 of vector stock per 1.2×105 cells.
In particular, the trimeric clamps of T4 and eukaryotes are less stable [ 85], and thus may require a subunit to bind both sides of the open interface to help stabilize the open form and to keep clamp protomers from dissociating.
Other questions are; how the repressed regions: the LADs in human cells and the gene clusters induced by nitrogen starvation in fission yeast are kept at the nuclear periphery, how do they dissociate upon transcriptional activation?
Freshly dissociated neonatal CMs were similarly plated and kept for 1 day in CM medium.
This could be due to differences in protocols: Shi et al. dissociated hPSCs, whereas Chatzi et al. kept hESCs aggregated.
They were heated at 60 °C (90 °C for CRP) for 15 min to dissociate the triple-helical form and then kept at 4 °C overnight (10 18 h) to refold.
An EMDP keeps perception and action embedded within sensorimotor schemes rather than dissociated, in compliance with theories of embodied cognition.
