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The archerfish used in this experiment were kept as described in Newport, et al.41.
Briefly, OLN-A53T cells were kept as described, harvested in PBS, centrifuged, resuspended in HEPES buffer (5 mM HEPES, 1 mM EDTA, pH 7.5) and sonicated.
Animals were kept as described previously.
The O. fasciatus (milkweed bug) culture was kept as described in Hughes and Kaufman [ 63].
The best extensions (i.e. those with the lowest Pext scores) are kept, as described in more detail in Section 3.4.
The flies were then separated into males and females and were kept as described in the fly rearing section (see Extended Experimental Procedures ).
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Groups of six fish were put into tanks and kept as previously described [ 61] at nine degree water temperature.
All other parameters in Differential Evolution are kept constant as described by Table 2.
Other conditions were kept constant as described earlier (Narra et al. 2012).
The placement and distance of the recording and stimulating electrodes were kept constant as described previously [37].
In brief, rats were anaesthetised and kept warm, as described above.
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