Unlike the constitutive expression from the psbA2 promoter, the nrsB promoter provides an inducible system, which may facilitate genome integration of potentially detrimental genes by keeping expression low until the time of induction.
There were fewer eQTLs in genes that are shared across a wide variety of species, possibly because keeping the expression of these genes stable is important for processes that are essential for life.
Data from maize and brassica further indicate that both gene fractionation, leading to extensive gene loss, and genome dominance are occurring simultaneously keeping the expression of the retained genes at elevated levels [ 16, 17].
As mentioned in the earlier section, MDM2 is an E3 ubiquitin ligase that induces polyubiquitination of p53 protein and subsequently promotes its proteolytic degradation in the 26S proteasome complex, thereby keeping basal expression of p53 at bay under normal conditions [ 41, 46, 47].
In keeping with this, expression of dll4, notch1b and the Notch targets hey2/gridlock and her12 were reduced by tmem33 knockdown using global CRISPRi at 48 hpf but not 26 hpf (Fig. 6g, h and Supplementary Table 3).
This is in keeping with the expression of polycystins throughout the embryonic and adult cardiovascular systems as well as in both endothelial cells and vascular smooth muscle cells [7], [8], [18], [26].
In keeping with this, expression of RAMP protein was higher in gastric cancer tissues compared with their adjacent non-cancerous tissues, whereas moderate protein expression were noted in intestinal metaplasia.
In keeping with reduced expression of enzymes of glycogenesis (Hex-C, Pgm, UGP, GlyS, and AGBE) and lipogenesis (sea, ATPCL, ACC, FASN, Gpdh, Gk, and mdy), unchallenged Mef2 knockdown flies were almost entirely devoid of triglyceride and glycogen.
The key to these successes for multi-subunit complex isolation is the idea of keeping the expression at its physiological level, rather than overexpression.
