Sentence examples for keep the supernatant from inspiring English sources
In the last wash we keep the supernatant and use this in control samples to ensure that no free label remains and can be demonstrated in micrographs.
Keep the supernatant and discard the pellet.
Keep the supernatant (solubilized proteins) Dissect 2 mice brains per condition and homogenize with a polytron in 10 ml PBS.
The resulting suspension was centrifuged at 4°C for 1 h at 2700 g, and the pellet was discarded and we keep the supernatant.
Keep the supernatant (solubilized proteins) Incubate solubilized proteins of 2 mice brains with immobilized GST fusion proteins overnight in rotation at 4°C Wash beads 5 times with 0.5 M NaCl Incubate beads with 10 mM reduced glutathione for 1 h in rotation at 4°C Centrifuge at 1,000 x g for 10 min at 4°C.
Then kept the supernatant on ice for 10 min and at −80°C for 1 2 h to promote precipitation of the perchlorate and centrifuged again.
Plant tissues (0.1 g) were ground in grinding buffer (0.5 ml) (50 mM sodium phosphate pH 7.0, 200 mM NaCl, 10 mM MgCl2 and 10%% glycerol, one protease inhibitor cocktail tablet/10 ml), vibrated and placed for 10 min on ice, then centrifuged at the maximum speed at 4 °C for 10 min and kept the supernatant.
The glycosylated protein strongly retains its enzyme activity; when we kept the fermentation supernatant at 4°C and sampled the lipase activity twice per month, we found no reduction in the activity after six months.
For chlorophyll-a and carotenoid determination, the pellet obtained was kept and the supernatant was discarded, and to the pellet, 5.0 ml of 25% absolute methanol was added and it was incubated at 50°C for 1 h.
Ten microliters of the upper yellowish liquid was removed and kept as the supernatant fraction.
