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Grouper juveniles were reared in 250-l circular fiberglass tanks maintained in a flow-through seawater system.
Juveniles were reared at the Lab of General and Systematic Zoology of the University of Greifswald in cylindrical plastic containers (5×10 cm).
Juveniles were reared with dry pellet feed and released from the hatchery to the river in the spring of the subsequent year at ca. 5 cm fork length.
After hatch, juveniles were reared in recirculating aquaria for one month at which time 100 individuals from each family were sampled as whole fry and stored in RNA later (Ambion, Inc., Foster City, CA).
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The juveniles are reared at this hatchery and then released as smolts, usually at the age of two years (Petersson et al. 2013).
Based on an analysis on European sea bass, a fish with a polygenic system of sex determination in which genetics and temperature contribute almost equally to the sexual phenotype [ 170, 171], Navarro-Martín et al. [ 138] reported that the methylation levels of the promoter of the gonadal cyp19a1 gene are sex-specific and influenced by the temperature at which one-year-old juveniles are reared.
Juvenile BKC were reared in four temperatures (1.5 to 12 °C) for a period of 60 days in low-density populations (150 crabs m− 2) and 120 days in individual cultures.
Juvenile three-spined sticklebacks were reared until sexual maturation on a diet based on antioxidant-free fish-feed pellets supplemented with either high (200 µg g−1) or low (10 µg g−1) levels of carotenoids (astaxanthin, lutein and zeaxanthin), but which were otherwise nutritionally identical.
The 'short' 5-HTTLPR allele has been associated with low serotonin metabolism and behavioural problems in infant and juvenile Rhesus monkeys reared with peers only, but not in monkeys who were reared with their mothers and peers during infancy.
Nematodes used for the experiment were reared on tomato seedlings of cv Early Urbana inoculated with 5000 second-stage juveniles (J2) as explained in Ebadi et al. (2009).
Larvae were reared using the standard protocol adopted [ 4, 5] and post-larvae and juveniles were fed a commercial diet (Biomar, Denmark, crude protein 63%, total lipids 14%).
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