Exact(2)
Embryos and larvae were grown under controlled conditions in the laboratory, and juveniles were maintained until use in this study.
Juveniles were maintained at the Washington State University Puyallup Research and Extension Center (Puyallup, WA) for the duration of the study.
Similar(58)
The juvenile fish were maintained at 25°C, 37‰ salinity and under continuous aeration.
Twenty days after their emergence from the egg sac, the spiderlings were housed individually in 1 litter plastic containers (16×8 cm ×8 cm) during the larval period (1 year and four moult) and, from the four juvenile moults were maintained in 8 litters glass box (27×18 cm ×16 cm), before finally being transferred to 14 litters plastic containers (32×22 cm ×20 cm) after the pre-adult moult.
The freshly excysted juvenile flukes were maintained in Ringer's Locke solution at room temperature.
The freshly excysted juvenile flukes were maintained in Ringer's Locke solution at room temperature for 5-6 days.
Male and female juvenile individuals were maintained in the laboratory in 10 L tanks of aerated, filtered, sea water at 18 ± 1°C.
Male juvenile birds were maintained in the aviary in their home cages with both parents until the age 44 45 dph, at which point they were transferred to sound isolation/recording chambers until they began to sing and baseline song was acquired.
During 50 days, 600 juveniles silver catfish were maintained in a water recirculation system and fed with experimental diets, three times a day.
In the first challenge, primary inoculum was prepared from the original 21% CsCl gradient band and injected into juvenile P. monodon that were maintained for 60 days before analysis and also for preparation of inoculum (using the same protocol used to prepare the primary inoculum) for a second challenge test.
Juveniles from all treatment groups were maintained at 7°C. Larval developmental staging was performed according to Herbing et al. [ 75].
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