Sentence examples for junction to avoid from inspiring English sources

Where possible, the expected product amplicon was designed to span an exon/exon junction to avoid amplification from potentially contaminating genomic DNA.

Many of the primers used for qPCR have previously been described [40] and are listed in table 1. Primers were designed using NetPrimer (Premier BioSoft) to have Tm of 60°C, and where possible, to cross an exon-exon junction to avoid amplification of genomic DNA.

Anterior structural support is often indicated in the lumbar spine and at the thoracolumbar junction to avoid kyphosis.

Almost all the primers used span an exon-exon junction to avoid DNA amplification (Supplementary Table 1).

OVATE FOR 3 and REV 2 was the primer pair used, with the forward primer specifically used due to its design in the first exon - intron junction to avoid amplification of genomic DNA.

They were designed manually to have primers spanning exon-exon junctions to avoid the amplification of DNA contaminant.

Primers were designed with the Primer Express software (Applied Biosystems) and selected so as to span exon-exon junctions to avoid detection of genomic DNA (see Table S1 – List of primers used in quantitative RT-PCR experiments).

All primer products span exon exon junctions to avoid amplification of genomic DNA.

This program supports primer design for one specific transcript at exon junctions to avoid unspecific amplification due to DNA contamination.

One of each primer pair (Additional file 1, Table S4) was designed to span exon-exon junctions to avoid amplification of genomic DNA.

The pattern of Gli-1 expression in this study was obtained consistently in different samples using quantitative RT-PCR and primers that span the exon exon junctions to avoid genomic contamination.