Although expression of the important tight junction components zonula occuldens protein (ZO -1, occludin and junctional adhesion molecule 1 (JAMA-1) was unaffected in the intestines of elderly individuals, claudin-2 expression was increased.
Expression analyses indicated that cell cycle factors mps1, cdc37, and PA2G4, and cell junction components cx43 and cldn5, are miR-133 targets during regeneration.
The diverse binding potential of ZOs is just one example of how cell junction components are dynamically dependent upon one another for functional integrity.
This demonstrates that defects in neuromuscular junction components can lead to non-neural developmental defects.
Myosin activation is also critical for positioning of tight junction components in cell monolayers derived from the human intestine [33].
Next, we examined the expression levels of adherens junction components in pRb-expressing osteoblasts stably expressing either dnCdk5 or RacV12.
Up-regulated genes chosen for validation included those coding for adherens junction components (α- and δ-catenins, cadherin1/E-cadherin/CDH1, nf2/merlin, cadherin-26, and OB-cadherin).
Staining for tight junction components in embryonic tendon and keratin 1 and 10 in postnatal tendon was a convenient method of visualising the tendon epithelium.
Any errors in this arrangement (Figure 2D, E) may indicate aberrant junction components or assembly, and were scored by allocating one point.
An epidermal barrier is in part formed by tight junction components which in adults are localized to a narrow zone of the granular cells [46].
Our survey of cell junction components, however, failed to detect any specific tight or adherent junctions highly accumulated in notochord plate cells (data not shown).
