Sentence examples similar to jumpstart reaction from inspiring English sources

As the sunlight hits this smog, it jumpstarts photochemical reactions on methane and other molecules, resulting in a complex mix of other, smaller hydrocarbons like acetylene and ethylene.

All PCR reactions had a final volume of 20 μL and contained 10 μL of 2× Jumpstart cyber green reaction mix, 0.2 μL 1 μM flourescein, 2.4 μL 25 mM MgCl2, 0.2 μL 10 μM forward primer, 0.2 μL 10 μM reverse primer, 2 μL template (20 ng/μL) and 5 μL sterile H2O.

Quantitative differences in gene expression were determined by PCR with JumpStart REDTaq ReadyMix Reaction Mix (Sigma) using the first strand cDNA as a template.

PCR was carried out in a total volume of 25  μL including 25 ng total DNA, 5 pmol of each sense and antisense primers and 12.5  μL JumpStart REDTaq ReadyMix Reaction Mix (SIGMA, Missouri, USA), which contains 20 mM Tris-HCl, pH 8.3, 100 mM KCl, 4 mM MgCl2, 0.002% gelatin, 0.4 mM each dNTP, inert dye, stabilizers, 0.06 unit/ μL Taq DNA Polymerase, JumpStart Taq antibody.

For the (CTG 240 or 480 transgenes, 1 µl (0.1 µg) of total DNA samples was amplified in 20 µl reactions using JumpStart™ AccuTaq™ LA DNA polymerase according to the manufacturer's instructions (Sigma) with 5% DMSO and 50 mM betaine.

Each 25 µL reaction contained 1× JumpStart REDTaq Ready Mix (Sigma-Aldrich, St .Louis, Missouri, USA), 100 nM of each primer, and 1 µL DNA extract (ca. 0.1 ng).

The PCR reaction was conducted following JumpStart™ Taq ready mix according to manufacturer's instructions (Sigma-Aldrich®) with the specific primers described in Table 2.

Resulting enriched DNA was PCR amplified for cloning in a 50-μL reaction containing 25 μL JumpStart REDTaq ReadyMix (Sigma-Aldrich), 800 nM ScaF linker, and 10 μL enriched DNA fragments.

Exonuclease I was inactivated by incubation at 80°C for 20 min. Linker-ligated DNA was PCR amplified in a 25-μL reaction containing 12.5 μL JumpStart REDTaq ReadyMix (Sigma-Aldrich, St .Louis, Missouri, USA), 800 nM ScaF linker, and 1 μL linker-ligated DNA.

All LAMP-BART reactions were performed in suitable nuclease free plastic tubes under molecular grade mineral oil, at 60°C for 90 min. Each 25 μl PCR reaction was performed using the JumpStart SYBR Green ready mix (Sigma) supplemented with 5 pmol of respective primers (a dedicated pair for each target; Table 1).

RNA was reverse-transcribed into complementary deoxyribonucleic acid (cDNA) by using an Affinity Script QPCR cDNA synthesis kit (Agilent Technologies, Santa Clara, CA, USA), and endpoint polymerase chain reaction (PCR) was performed with JumpStart Taq DNA Polymerase (Sigma-Aldrich) and primers for neurotransmitter receptors listed in Additional file 1: Table S1.