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The isothermal crystallization was investigated also by carrying out the temperature jump experiment of DSC thermograms, giving almost the same results as the infrared spectral measurements.
In the following, in analogy to a temperature jump experiment, MD simulations of the TRPZ1 peptide with explicit treatment of the solvent have been performed raising the temperatures of the system at values above 373 K.
Finally, motivated by earlier reports on strain rate influences, 9 we investigate the effect of an increased strain rate on the stress versus strain behavior of nanoscale tensile samples by a strain rate jump experiment.
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Firstly the repassivation has been studied by potential jump experiments.
Strain rate jump experiments show that the instantaneous strain rate sensitivity in the semisolid state is similar to that of the solid phase.
The activation volumes determined from strain rate jump experiments and from stress relaxation for crystals thicker than 40 nm are nearly constant at a level of 8.1 nm3.
The time course of the collapse could not be resolved by pressure or temperature jump experiments, indicating that the collapsed and extended forms are not separated by an energy barrier.
The aim of this work is twofold: On the one hand, design criteria of pH jump experiments are discussed, on the other hand, the folding mechanism of poly-l-glutamic acid is clarified by probing the IR response of the amide I band.
Temperature jump experiments showed that stacking and unstacking in polyC and CpC occur on the time scale of 100 ns.
Experimental approaches to measure folding kinetics in detail, such as temperature jump experiments or single molecular methods [ 7], can be employed to check the computational model and predictions, in turn, can be pivotal to RNA rational design.
The overall refolding rate following urea dilution is increased 2.5-fold over that of the wild-type protein, and the rates of the individual Ia → Ib and Ib → N steps, observed in pH jump experiments, are each increased 20 30% by the disulfide.
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