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This test uses whole blood-derived RNA and PCR primers annealing to the leader regions and the joining region segments.
On the basis of these sequences, we designed 6 forward primers at the variable region and 5 reverse primers at the joining region.
The maximum strain and stress occur at the concave arc joining region in which the thickness variation of the two joined tubes is small.
However, it shows some level of disorganization in the MPER-TM joining region due to the tendency of R696 to interact with the polar membrane surface.
To reduce the number of PCR reactions, we constructed a multiplex PCR analysis system using a mixture of IGHV-1 and IGHV-3-specific primers as forward primers and a mixture of 5 joining region reverse primers.
Heemskerk, M.H. et al. Redirection of antileukemic reactivity of peripheral T lymphocytes using gene transfer of minor histocompatibility antigen HA-2-specific T-cell receptor complexes expressing a conserved alpha joining region.
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Local splitting damage at cell joining regions and crushing of the cell of the egg-box structure was identified as the primary failure mechanism in the sandwich panels.
The introduction of these additional transverse terms requires a specific treatment for the computational domain's edges and corners, as well as a suited set of compatibility conditions for boundaries joining regions associated to different flow properties, as inlet, outlet or wall.
Totally we found 824 T regions in genes that were extended via joining regions T1 and/or T2.
In order to find a single region-of-interest at the same genomic location, these overlapping regions are integrated by joining regions with one or more overlapping reads.
The classical protocols to join DNA fragments by restriction and ligation as well as most techniques employing recombination depend on the presence of specific short sequences at or around the joining regions.
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