Exact(10)
We previously developed a synthetic cis-acting RNA ligase ribozyme with 3′ 5′ joining activity termed "DSL" (designed and selected ligase).
Surprisingly, the infamously high 'non-homologous end joining' activity of K. marxianus enabled simultaneous integration of all 4 genes in a single step, with a probability of 7.9%.
We thus tested the GRC efficiency in a lig4Δ mutant strain where non-homologous end joining activity is deficient [10].
Participants reported cost, childcare, lack of time and low awareness as barriers to joining activity classes.
Overall, the single and multiple mutants displayed stimulated strand joining activity with increased concentration of PEG 8000.
We next examined whether the conservative active site mutations that we had introduced changed the pH optimum for intermolecular strand joining activity (ligation step 3).
Similar(50)
The 'it' only becomes problematic when the individual is unable to re-engage in a productive, rewarding life - joining activities and socializing.
By joining activities, you are also putting yourself out there to communicate with people with the same interests.
Joining activities at school will make you busier so you won't have time to think about home.
Tellingly, neither enzyme cleaved the 5′-flap of this substrate to generate shorter products (left hand lanes), confirming that Cas1 catalyses a concerted TES reaction rather than a sequential 'cut and join' activity.
Residents — generally in their 80s — are mobile enough to eat in the dining hall and join activities.
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