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The significant similar tracts identified were then aligned and joined to construct the putative Atlantys LTR.
Next, the two matrices are joined to construct the hybrid similarity matrix.
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The UCSC Table Browser function [ 44] was used to retrieve relevant sequences for subsequent joining to construct full-length ORF models for the different gene loci.
After the final donor DNA fragments, which included vector sequences, were joined to the construct that contained the array of fragments, the ligation product (the construct) was thereby released from the beads via digestion with a rare-cut meganuclease; the freed linear construct was circularized via an intra-molecular ligation.
China should shake off its old self and join in to construct a truly better sustainable, longer lasting and peaceful world.
One video documents artists and volunteers joining forces to construct a shimmering mural made of reflective glass, a radiant symbol of the Mexicali community's efforts to let in the light.
If the CCD option is selected for one of the LC detector systems, it is hoped that these groups will join forces to construct the new detector.
The small islands of Burray and South Ronaldsay, to the south of East Mainland, are now joined to it by causeways constructed during World War II to prevent enemy submarines from entering the naval base at Scapa Flow.
Other substitution models and neighbor-joining methods to construct phylogenetic trees produced very similar trees (H. zu Dohna, unpub. data).
The final distance matrix created with ClustalW2 was then used with the neighbor-joining method to construct the phylogenetic tree and the final tree was plotted as a phylogram.
To distinguish between a single primary (clonal) infection and repeated, independent infections, we determined the distance between every two isolates based on their SNP profile and used as a heuristic a neighbor-joining algorithm to construct a phylogenetic tree from this distance metric ('Materials and methods', Figure 2B).
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