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Where possible, Newbler assembly sequence was added manually and gaps in the draft were joined manually.
These regions were joined manually, and they were then verified to be chemotype-specific by a local BLASTN search.
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At branch points, the centerlines were joined together manually to complete the wiring circuitry of the axons.
The manual-transmission Chevrolet SS joins other manually shifted sporting models such as the Buick Regal GS mid-size sedan and compact Ford Fiesta ST hatchback – the latter only available with a six-speed stick.
One is to join variables manually.
These were manually joined together.
We ensured that the connections between manually joined contigs were supported by paired-end read mapping.
These were visually inspected using SeqMan (DNASTAR Lasergene) and manually joined when appropriate.
The assembly for NCYC3585 produced two major contigs that overlapped (identified using MUMmer 3.0 [ 67]) by ~3000 bp and were manually joined to form a single scaffold.
After alignment to the UMD3.1 in silico BamHI restriction map, they were manually joined into chromosome-wide optical maps and viewed using GnomSpace --a map- centric genome viewer that facilitates inspection of alignments.
The protein clusters were manually joined into groups; despite several attempts at clustering and phylogenetic analyses, some sequence groups could not unambiguously be grouped with TamB or AsmA, and are therefore labeled as AsmA-TamB hereafter.
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