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The Zn isotopic results in this study are given in the recommended delta notation for the 66Zn/64Zn ratio: δ66Zn = [((66Zn/64Zn)s / (66Zn/64Zn JMC -1]*1000 (in ‰) where s stands for sample and JMC represents the Zn isotopic standard solution (JMC 3 0749 L) [55].

Figure 2 Isotope plot obtained during measurements of the samples using δ6XZn= [((6XZn/64Zn)sample / 6XZn/64Zn Lyon JMC 3-0749L)- 1] * 1000 (in ‰).

Solid and dashed lines respectively represent the average 176Hf/177Hf of JMC 14375 and 2σ S. D. on the mean for the unspiked solution.

The basic instrumental capability of the KBSI Neptune MC-ICP-MS was tested by using a JMC 475 Hf standard solution with a concentration of 200 ng ml-1.

We also measured Hf isotope ratios of in-house standard solution JMC 14375, delivered from Alfa Aesar of Johnson Matthey Company (stock no. 14375, lot no. 83-084740F, plastandarddard solution).

Figure 2 The 176 Hf/ 177 Hf isotopic measurements for a mixed standard solution of which concentrations were 298.7 ng ml -1 for JMC 14375 Hf, 30.4 ng ml -1 for Accu-Trace Yb and 3.0 ng ml -1 for Accu-Trace Lu. Accu-Trace Lu

The 176Hf/177Hf ratio of this standard solution (300 ng ml-1 Hf), measured with the same analytical design as that for the measurement of JMC 475 standard solution (20 cycles, 4.194 s integration) gave an average of 0.282228±0.000005 (n=10, 2σ S. E). (Table 3).

The β(Hf) and β Yb) values were measured by monitoring 179Hf/177Hf and 172Yb/173Yb ratios for a mixed standard solution of which concentrations were 298.7 ng ml-1 for JMC 14375 Hf, 30.4 ng ml-1 for Accu-Trace Yb (lot no. B4035064-2B, reference standard) and 3.0 ng ml-1 for Accu-Trace Lu (lot no. B8045141, reference standard).

Figure 3 Relation between the Hf and Yb mass bias factors ( β (Hf) and β (Yb)) for the same mixed standard solution as that described in Figure 2. We further tested the validity of isobaric interference correction described above by using Hf+Yb+Lu solutions mixed with different elemental proportions (Hf = 300 ng ml-1 JMC 14375, Hf Yb Lu ≈ 200 10 1, 100 10 1, 50 10 1, 30 9 1).

In previous study, we have built a model for the active conformation of human 5-LOX, and identified naphthalen-1-yl 3,5-dinitrobenzoate (JMC-4) as a 5-LO Xinhibitor by virtual screening.

All animals were handled in strict accordance with good animal practice as defined by the procedure used (CEEA JMC-07-1001-CPC), whash has been approved by the institutional animal care and use ethic committee (PRBB-IACUC).

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