JC worked out the protocol, developed searches and data management, will participate in selection, inclusion, quality assessment and data extraction of papers for several reviews, will perform statistical analyses and wrote the first draft of the manuscript.
JC is working as an assistant professor of Physics at the SSN College of Engineering, Kalavakkam, Tamil Nadu, India and has been involved in polymeric materials research.
JC therefore worked closely with APHRC's communications team in designing and implementing research communication related to sexual and reproductive health research.
In brief, 10 cells were washed and resuspended in 500 μL of JC-1 working solution.
Samples were added with 0.5 ml JC-1 working solution and incubated for 20 min at 37 °C in the dark.
Tow hours after SDT, the cells were then washed with PBS and incubated with JC-1 working solution for 20 min at 37°C in the dark.
Briefly, 48 h after siRNA transfection, the cells were collected and resuspended in 500 μl JC-1 working solution for 20 30 min.
Eight of them were sampled on different days in order to evaluate the overall reproducibility of Δψm measurements, i.e. the constancy of the orange/green fluorescence ratio when day, JC-1 working solution and laboratory handling are changed.
JC guided the work, supervised the experimental design, and helped in drafting the manuscript.
JC: Because my work is content based, I go from content to form, that is, I select a medium or technique that seems most appropriate for what I want to express.
