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The oxidation state of PtNPs was determined by X-ray photoelectron spectroscopy using an X-ray recorded on a PHI 5000 VersaProbe (ULVAC-PHI, Inc., Chigasaki, Japan) system using a micro-focused (100 μm, 25 W) Al X-ray beam.
Then the lipopeptide was dissolved in deionized water for further purification using a Shimadzu LC-20A high efficiency liquid chromatography (HPLC) (Shimadzu, Japan) system quipped with a C18 column and detector at 280 and 215 nm.
The Si wafers were then dipped in dilute hydrofluoric acid to form a passive surface layer, which allowed the wafers to maintain their clean surfaces when transported through air before being introduced into the loadlock chamber of the ultrahigh vacuum chemical vapor deposition (UHVCVD, ANELVA SRE-612 Japan) system [13, 14].
HPLC (VWR-Hitachi LaChrom Elite® -Tokyo, Japan) system was equipped with a Pump L-2130 auto-samplerpler L-2200 from Hitachi (Tokyo, Japan), oven Gecko 2000 (Hattersheim, Germany) set at 40°C and refraction index (RI) detector L-2200 (Hitachi-Tokyo, Japan).
Transmission electron microscopy (TEM) images were acquired on a JEM-2010 TEM (JEOL, Japan) system.
Plasma presepsin levels were measured by a chemiluminescent enzyme immunoassay (Pathfast, Mitsubishi Chemical Medicine Corporation, Japan) system.
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