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Cases were classified as positive if the overall CA IX score was ⩾50.
Figure 3 shows scatter plots of the relationship between CA IX score and these two predictive variables.
Carbonic anhydrase IX expression was evaluated by analysing the CA IX intensity, % of positive tumour cells, and CA IX score.
No significant correlation is seen between CA IX score and either HER2 ratio or TOP2A ratio (n=140).
Glucose transporter 1 (GLUtranscriptiontion fold changes on the in vivo array showed a significant correlation with the immunohistochemical CA IX score as a surrogate marker of hypoxia.
The product of the intensity staining and the percentage of tumour produced a final immunostaining score (CA IX score) of 0 300.
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CA IX scoring was positive in three invasive cancers and 13 superficial tumours.
GLUT-1 fold changes correlated with CA IX scores (P=0.008 r=0.21, Figure 3), and were significantly higher in CA IX-positive tumours (means 0.9 vs 1.8 P=0.03 two-tailed t-test variances not assumed to be equal).
Correlation of tumour CA IX staining scores with gene fold changes for all eight genes individually and summed was tested using linear regression.
For GLUT-1, CA IX and Ki67, score 0, no staining ('absent'); score 1, <10% positive ('weak'); score 2, 11% to 50% positive ('moderate'); and score 3, >50% positive ('strong'strong
Japan's (and probably the world's) most renowned gaming magazine, the Famitsu [JP], has given Square Enix's RPG Dragon Quest IX a perfect score in the latest issue.
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