The apical system of the genus Collyrites is subdivided in two parts: (i) an anterior part composed by four genital (1, 2, 3, 4) and three ocular (II, III, IV) plates, called trivium; (ii) a posterior part composed by two ocular plates (I, V) and one genital plate (5), without gonopor, which circle the periproct, called bivium.
For details of DNA transfections, collagen IV plating, laminin competition, co-immunoprecipitation, and immunofluorescence experiments, see Supplemental Experimental Procedures.
The four compound sets screened include: the "Approved Oncology Drugs Set IV" (plates 4762 4763) which consisted of 101 compounds; the "Diversity Set" (plates 4770 4789), which consisted of 1597 compounds; the "Mechanistic Set" (plates 4742 4752), which consisted of 879 compounds; and the "Natural Products Set II" (plates 13120880 and 13120881), which consisted of 120 compounds.
Sera were isolated from mice via cardiac puncture and centrifugation at 15 000× g for 15 min. To assess parasite-specific IgG1 and IgG2a, Immunlon IV plates (Thermo Life Sciences) were coated at 4°C overnight with 50 μg/mL of T. muris E/S in carbonate/bicarbonate buffer, pH 9.6.
All ESTs in a given library shared the library stem, batches generally contained four plates (I-IV), and 80% of plates were sequenced from both 5' and 3' directions.
on an IV N plate taken on Mar.
Diffraction data of WT0 and WT1 were collected utilizing Rigaku RAXIS IV image plate detector at Institute of Biophysics (Chinese Academy of Sciences).
The HPTLC system (CAMAG, Muttenz, Switzerland) consisted of (i) Linomat 5 sample applicator using 100 μL syringes, connected to a nitrogen tank; (ii) ADC 2 chamber containing twin trough chamber 20 × 10 cm; (iii) immersion device III; (iv) TLC Plate Heater III; (v) TLC visualizer; (vi) TLC scanner 3 linked to winCATS software.
In a novel differentiation protocol, subconfluent hESC were dissociated with collagenase IV and plated in ultra low adherence 6-well plates for sphere formation at a density of 200,000 cells/cm200,000
Data were collected at 100K using an R-AXIS-IV image plate detector mounted on a Rigaku 200HB generator.
