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Combination of RAPD and ITS typing gave an index of discrimination of 0.844.
ITS typing employing primers designed for conserved eubacterial rRNA sequences gave only two ITS types, and was therefore not suited for genotyping of L. monocytogenes.
ITS typing revealed a 21.2% hospital misidentification rate.
The first ITS typing system was developed by Lu et al. (22 ).
Therefore, we performed the 16S-32S intranscribedspacerd spacer (ITS) typing [ 16].
The samples were chosen from each ITS cluster shown in Figure 1 and MLST was performed in order to compare between MLST and ITS neighbor-joining trees to determine whether ITS typing alone could be used to reflect strain relatedness.
Similar(41)
Richness of ITS sequences was high (239 ITS types were detected).
Of its type it was a classic.
It's one of the most successful schemes of its type – five others modelled on it are in the pipeline.
Was this the first instance of its type and or has it ever been repeated?
"It's probably the last of its type," said Spindler.
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