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Figure 5 indicates that our ITS data largely correspond to the previously published ITS sequences of Zea, although there are a few samples that are not always well grouped.
All ITS sequences determined during this screening study are different from the known ITS sequences of the other Nematosporaceae species shown in the phylogenetic tree of Figure 1.
These studies and our findings in ITS sequences of Berberis indicate none of the tested three genera have paralogs of ITS.
To build these four alignments, we first used the ITS sequences of full length, including any fringe residues of 18S and 28S, to construct "whole ITS" alignments with both Clustal W and SATé.
The incorporation of ITS sequences of human pathogenic Candida species into public nucleotide sequence databases and the reliability of ITS sequence data were also addressed.
A host of single nucleotide polymorphism (SNP) sites were detected among ITS sequences of five Citrus species.
The ITS sequences of 91 isolates of P. noxius from Taiwan were analyzed in order to understand the phylogenetic relationship among these isolates.
The ITS sequences of 36 isolates of P. noxius, representing all ITS types found in Taiwan, were submitted to NCBI National Center for Biotechnology Informationn; http://www.ncbi.nlm.mih.gov).nlm.mih.gov
From about 50 ITS sequences of H. setigerum available in GenBank, we selected those representing the main clades within this species complex (Nilsson et al., [2003]).
Among the three ITS sequences of T. japonicum cited below, AB027366 and EU039882 are from IFO 9647, while JN049824 is from OSC 110991.
Genus-specific Taqman probes were designed from the ITS sequences of the most important mycotoxigenic species of Fusarium, Penicillium, and Aspergillus.
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