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The ITS regions are located between the 18S and 28S rRNA genes and the rRNA gene for 5.8S RNA separates the two ITS regions.
We isolated genomic DNA sample from the strain and sequenced the amplified ITS regions.
PCR assays targeting the ITS regions in ribosomal DNA were designed to identify and differentiate species.
Fungal identification was based on sequence analysis (GenBank Accession No. MG739623) of internal transcribed spacer (ITS) regions of the rDNA.
Coding regions of rDNA are highly specific to species but ITS regions vary due to insertions, deletions, and point mutations.
Molecular identification of isolated mycelia was performed using sequence data of the ITS regions of the ribosomal DNA.
The genetic diversity within 18S rRNA gene and ITS regions sequences of various obtained Theileria species (spp).
We used ITS regions of ribosomal rRNA genes and morphology to confirm its identity.
The ITS regions are also valuable for studies of fungal communities [9] and DNA barcoding [10].
Reconstructed contigs of ITS regions from 454 data were characterized by high read depth (Figure 2).
Both Sanger sequencing of amplified ITS regions and whole genome 454 sequencing lead to similar phylogenetic inferences.
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