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We used sequences of the nuclear ribosomal DNA ITS region to infer phylogenetic relationships.
The ITS region in rDNAs comprises of ITS1 and ITS2.
Further confirmation was done by PCR amplification of ITS region.
Sequence data of the ITS region was submitted to the DNA Data Bank of Japan (DDBJ).
The ITS region of heterogeneous diploids and all hybrid clones was cloned and 15 85 DNA clones bearing ITS region were sequenced (Table S1).
PCR assays amplified the ITS region.
Here, we explore the ITS region instead.
Their presence was limited to <0.5% of the ITS region.
Sequencing of the nuclear ITS region revealed 12 polymorphic sites.
Amplification of the ITS region was performed according to [ 70].
The ITS region was amplified by nested PCR.
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