"Blue ribbon visualization" is an in vivo lineage-tracing technique that uses the LacZ reporter gene under the activity of a promoter, allowing the identification of a cell of interest and its tracing in potential progeny.
Parentage assignments based on microsatellite allele information could lead to the identification of appropriate parents contributing for potential progeny (Grattapaglia et al. [2004]a).
We then use a formal approach based on mathematical models describing the dynamics of population size evolution to test potential progeny links and demonstrate that most cells follow a linear naive→early effector→late effector→memory pathway.
Potential progeny of the CBC cells would not express GFP, but the activated LacZ reporter would function as a lineage tracer.
To label rk pan -gal4 expressing cells and their potential progeny, we used the G-TRACE lineage-tracing system [ 16] to independently label rk-positive cells (rk pan -gal4 ts /UAS-RedStinger) and their potential progeny (rk pan -gal4 ts /UAS-Flp/Ubi>stop>eGFP) over time.
Because there is huge growth of the population during the early generations, we can only analyze a small fraction of the potential progeny (we cull the population at each generation).
Even if Nlp malfunction in MT nucleation and spindle formation undoubtedly fosters multinucleated progenies, we still need to explore its potential direct contributions to cytokinesis.
Commerce lags behind its potential.
