Exact(48)
In addition, the 'universality' of the use of ITS marker in Anopheles studies makes it easy for other researchers to relate directly their work with our results.
In this way a specific hazelnut primer pair based on the ITS marker (70 bp) and a nuclease (TaqMan) probe labelled with FAM and BHQ were designed.
The ITS marker is one of the few markers shown to be divergent between the An.
As was observed for the ITS marker, the Brazilian samples of A. germinans presented a more complex phylogeographic pattern.
Here, we applied the same ITS marker to study genetic changes in Caullerya populations in more detail.
Here changes in species-specific demographic histories over time were assessed using Bayesian skyline analysis [ 138] in BEAST v1.6.1 [ 23] using the ribosomal ITS marker.
Similar(12)
The multiplex PCR assays were further established for the joint use of some SCAR and ITS markers efficiently.
Species identification within the Ulva clade requires high resolution DNA markers and/or hybridization experiments and is not possible by reliance on the ITS markers alone.
We sequenced chloroplast rbcL, matK, and nuclear ITS markers, and analyzed them with Most Parsimony and Bayesian Inference.
The 28S and ITS markers, however, were much longer (981-993 bp and 1589-2100 bp, respectively) and additional sequencing was performed using internal primers (Table 3).
Microsatellite analyses revealed clear genetic differentiation between western (temperate) and eastern (subtropical/tropical) populations, confirming divergence previously detected with mitochondrial (COI) and nuclear (ITS) markers.
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