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DNA metabarcoding of complex bacterial and fungal communities by profiling of 16S rDNA sequences and internal transcribed regions (ITS) had opened new prospects in studying and designing of new efficient probiotics based on fermentation process.
Of the eight plant DNA markers we tested (rbcLa, rpoC1, rpoB, matK, ycf5, trnL, psbA-trnH, ITS), matK and ITS had a low rate of sequencing success.
ITS had an excellent performance in recovering monophyletic species, but this represents a biased sample, as we could amplify ITS for less than half of the individuals.
In comparison, ITS had a relatively lower efficiency at 75%.
There were no shared mutations in the β-TUB gene; GPDH and ITS had a near equivalent number of shared mutations and ACT had the least number.
ITS had an identification efficiency superior to that of ITS2 in the following 12 genera: Gunnera, Luzula, Strobilanthes, Nepeta, Dionysia, Adenia, Clidemia, Sedum, Indigofera, Kalanchoe, Pilea, and Melampodium.
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It had its charm.
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I can imagine its having a resurgence".
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