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The primer pair and PCR condition of ITS followed the cited reference (White et al. 1990).
The highest variability was obtained for ITS, followed by psbA-trnH, trnL and matK.
Irrespective of the clustering algorithm, the best rate of correct assignment was obtained for ITS followed by matK, psbA-trnH, rpoB, rbcL and trnL.
To determine the taxonomic affinities of these strains, primers ITS5 and ITS4 [ 21] were used to amplify the approximately 540bp ITS, followed by sequencing with the same primers.
Its ironic lyrics underscore the low expectations...We Love Malcolm, repeats until its followed by the refrain, cause no one else does.
Like misremembered school bus tragedies and rumors about candy tampering, Trick 'r Treat has managed to incorporate itself into Halloween observances through word of mouth, but its followed a rocky path to get to this point.
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CEO of Professional Science Editing for Scientists @ prosciediting.com