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This result, in conjunction with the accumulation of LC-3 II, suggests that RB silencing blocks VP-16-induced autophagic flux in its final steps.
The literature shows that autophagy may serve as a resistance mechanism in tumor cells, including GBM, as the pharmacological inhibition of its final steps increases chemotherapy-induced apoptosis.
Katayama et al. showed that the pharmacological inhibition of the autophagic process, in its final steps, increased the apoptotic rate induced by the compound.
To test whether RB knockdown in fact blocked VP-16-induced autophagic flux in its final steps, we performed western blotting experiments to detect p62 levels.
Indeed, in vitro, the experimental synchronisation of the mitotic cell death triggering obtained by the cell electropermeabilisation, which allowed the study of the initial steps of this pathway, could not be maintained to describe its final steps.
From the original conceptualizing and initiating this project, to its final steps, Rina Gill has been instrumental in providing leadership and guidance through the many stages of its development.
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Mr. Perry applauded lawmakers for passing the bill, saying, "Today the Texas Legislature took its final step in our historic effort to protect life".
As I previously wrote, the production is in its final step.
Eventbrite has taken its final step toward becoming a publicly traded company.
The transport model of observed area is perceived in its final step.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com