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Upon cell binding to the ECM, the association of RIN2 with R-Ras-GTP lessens its Rab5 GEF activity and maximizes its docking function.
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We tested different Rab3a conformations and genetic interactions with the SM-gene munc18-1 on the docking function of Rab3a in mammalian chromaffin cells.
Munc18-1 has a docking phenotype in many systems including C. elegans [52], [53] and, although this docking function is thought to involve syntaxin binding, syntaxin-independent interactions may well contribute.
However, previous observations argued against a docking function for SNAP-25 (Sørensen et al. 2003).
This docking function could be achieved either through a direct physical interaction with Rab8 or, more likely, through participation in a complex of ciliopathy proteins required for Rab8 localization.
It is known that Munc18-1 interacts with syntaxin-1 and we therefore assumed that Munc18-1's docking function depends on syntaxin-1-interaction (de Wit et al. 2006; Gulyás-Kovács et al. 2007).
Taperin is expressed as multiple splice variants, and bioinformatic analyses indicate that taperin appeared during the vertebrate lineage, strictly maintains the PP1 docking function across vertebrate species and has an ancestral relationship with the PP1- and actin-binding protein, phostensin.
Different scoring and docking functions consequently favor different binding situations.
For an anti-protease drug to be active, its docking must block a vital protease function.
Both of these aspects of the dock function as one might hope – the card reader reads cards dependably, and the USB 2.0 hub.. hubs.
It was suggested that the requirement for Tom70 is related to its known function as a docking site for cytosolic chaperone proteins (Fan et al. 2011; Young et al. 2003b), which would likely be associated with aggregation prone precursor proteins.
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