Sentence examples for its added polyA from inspiring English sources

Presumably the sequence reads are from an insert of the chromosome 14 L1 that has transduced flanking sequence, and its added polyA tail.

Reads for the MAQC datasets were mapped onto Ensembl known transcripts with added polyA tails of 200 bp, also using TMAP.

Sequence analysis identified 20 nucleotides immediately adjacent to the added polyA nucleotides that aligned with the 3′ end of another lineage 2 strain of WNV, WNV-956, suggesting that this sequence represented the exact 3′ end of WNV-MAD78.

For assessing transcriptome quantification accuracy Ion Torrent reads from cancer datasets were mapped on the External RNA Controls Consortium ERCC RNANA spike-in controls reference [ 38] with added polyA tails of 200 bp using TMAP.

Upon arriving at the next intergenic junction, the polymerase stutters to add polyA, stops and restarts to synthesize the next gene.

We therefore conclude that a polyA effect is not readily apparent in these data, presumably because we had added unlabeled polyA to the hybridization mixture as a blocking agent.

To reduce the polyA effect, it is therefore common practice to add unlabeled polyA to the hybridization mixture to bind to the polyT segments of the hybridization probe, thus competing with the polyA segments in the microarray spots.

To reduce the polyA effect, it has become common practice to add unlabeled polyA to the hybridization mixture, to bind to the polyT segments of the hybridization probe, thus competing with the polyA segments in the microarray spots.

Its lots added up to $224.17 million.

The polyA tail is added at the polyA site (PAS) in the nucleus in a 2 two-step reaction consisting of a large cleavage complex that cleaves the pre-mRNA into two fragments followed by polyA tail addition to the upstream fragment [ 25].

Relative miRNA expression levels were quantified using the NCode miRNA first-strand cDNA synthesis kit (Invitrogen) to add a polyA tail onto the miRNAs.