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It will be difficult to exclude the possibility that cells carrying copy number alterations of growth-promoting or tumor suppressor genes have malignant potential by studying them in model organisms.
However, even with higher statistical power, it will be difficult to exclude the possibility that more Sockeye Salmon with strong antiviral responses survived the initial IHNV challenge than do those with weak responses and subsequently the surviving fish are those with the strongest antiviral response whereas the naïve fish would be a mixture of weak and strong responders.
However, generally when planning secondary research projects, there will be study results already available, either from publications, reports, or from other groups having access to the data, so that it will be difficult to exclude post hoc definitions of research objectives, for example, resulting in hunting for "statistical significance".
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