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It was quantitated by a colorimetric assay using LPO-586 (Bioxytech; OXIS International Inc., Portland, OR, USA), which after reacting with MDA, generating a stable chromophore that can be measured at 586 nm (Hitachi U2000 Spectro; Roche).
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The authors need to explain how they define processivity and PT stimulation differently and how it is quantitated.
When the expression was quantitated though, it was obvious that the transgenes that were in the opposite orientation to the ROSA26 promoter showed higher expression than those which were in the same orientation as the ROSA26 promoter (Figure 3C).
FRET was quantitated using an Olympus IX83 inverted fluorescence microscope.
Each sample of cDNA was quantitated and diluted to a similar concentration of 10 ng/mL.
Top, COX-2 mRNA was quantitated by real-time PCR using GAPDH as internal control.
Aortic intimal thickening was quantitated in H&E-stained segments.
In addition, trypan blue (TB) was quantitated by light microscopy.
Serum OPG was quantitated using enzyme-linked immunosorbent assay.
Bound [3H]9-cis-RA was quantitated by liquid scintillation counting.
Radioactivity was quantitated using a γ-counter.
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