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Kelihos was used for sending out spam and spreading malware until it was "neutralised" in September 2011.
The Kelihos botnet was used for sending out spam and spreading malware until it was "neutralised" in September 2011.
As TCA also inhibits the downstream assay, it was neutralised with Tris-acetate (pH 7.75) to bring the total TCA percentage to 0.1% following the manufacturer's direction.
Then it was neutralised with 1 M HCl to pH 6 8, an equal volume of 0.1 M Tris-HCl buffer was added and the sample was heated for 30 min in a boiling bath.
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"One was neutralised and the other was cornered," he said.
Wales had no plan B once Gatlandball was neutralised.
Molins said on Wednesday he was confident "a new team of terrorists was neutralised.
The eluate was neutralised with 1 M ammonium acetate.
The solution was neutralised with HNO3 before the measurements.
After 12 hours, the reaction was neutralised with hydrochloric acid and extracted with ethyl acetate (3 times).
The eluate was neutralised with ammonium acetate and incubated with THP-mal-J591c-scFv at room temperature.
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