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In addition, another type of microorganism which produced a chlorophyll-like compound was only found in one layer but it was detected using both laser wavelengths.
It was detected using an upstream PCR primer (CR25) following gene specific RT.
It was detected using FITC active caspase-3 apoptosis kit (BD Pharmingen, USA) according to the protocol provided.
This latter segment was exceptional as it was detected using comparative information from BAC clone 1B03 that has been fully sequenced [ 56].
In addition, α-actinin was demonstrated on astrocytoma cell surface where it was detected using α-actinin antibodies (Spehar and Strand, 1995).
Actin was used as the protein loading control and it was detected using monoclonal anti-actin antibody (ICN biomedicals, Burlingame, CA, 1 1000 dilution) and HRP conjugated anti-mouse secondary antibody (KPL, 1 5000).
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EGFRvIII is produced by tumors, so it is detected using western blotting [77].
It is detected using mouse primers DIC2_Ex 1b for and DIC2_R rev, and DIC2_Ex 1b for and DIC2_iso24 rev.
However, I found it extremely surprising that it is detected using HMM.
Siliciano and colleagues analyzed a group of patients who had taken powerful cocktails of HIV drugs that for 5 to 7 years clobbered HIV so successfully that it never was detected using the standard tests.
GFP fluorescence was detected using confocal microscopy.
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