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It was attained through ATP site-dependent binding competition assays and constitutes the first available dataset of this kind.
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Regardless of what mechanism the "enzyme"/ gene" trade-off is achieved by, it can be regarded as the first, prebiotic evolutionary step towards a simple form of phenotype amplification (the production of many enzymes from a single gene) – a feature of all recent organisms in which it is attained through transcriptional and translational amplification.
Consensus on methodological approaches was attained through vigorous debate.
Optimal resolution for extracting trees was attained through fusion of multi resolution (spectral and spatial) data.
In this study, methodological rigor was attained through verification (Meadows and Morse 2001).
Cross-site fidelity among those individuals serving as facilitators was attained through three mechanisms.
The equilibrium adsorption was attained through 20 min for both cadmium and copper metal and the equilibrium adsorption were determined at 80 and 68 mg/g (Fig. 4).
Validity was attained through a triple entry table that took into account investigations related to the study's categories which allowed individual triangulation.
No maximally tolerated dose of rapamycin was attained through escalation to the maximal planned dose of 0.08 mg/kg (2.5 mg/30kg dog).
By exchanging the concentrated ammonium hydroxide base solution at 72 h with the dilute solution, constant nitrogen restriction was attained through most of the production period (Figure 7a).
Credibility was attained through carefulness in selection of the context, the informants and data collection.
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