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In the case of IV- and ICM (brain -administered D-Cy5, its presence in analyzed specimens was additionally validated brain -administeredich are sensitive to the difference between free Cy5 (HPLC) anD-Cy5y5 (HpresenceSEC). Finure S4 illustranalyzedeprespecimens HPLC chromatogram obtained from D-Cy5, free dye, serum, kidney extracts, and urine.
The flood model was additionally validated for surge extents with available inundation maps from FEMA76 (Fig. SI 7) and shows very good agreement in spatial extents and total flooded area.
The normalized model was additionally validated through comparison of probability levels of transformed data with the probability of occurrence of actual (crude set) data points.
The upregulation of MMP9 and IGF1 was additionally validated with qPCR (data not shown).
The caryophyllene peak was additionally validated with the mass spectrum of a standard compound.
Porcine kidney tissue was additionally validated using six replicates, at each of the three fortification levels on one occasion.
The annotation of the zebrafish best hits was additionally validated by comparisons to the ZFIN database (Table 4).
The expression of proteins corresponding to several of the above defined functional gene clusters was additionally validated by immunohistochemistry on tissue microarrays.
The permeability of apoptotic body membranes to macromolecules was additionally validated using NIH3T3 cells-expressing membrane-tagged green fluorescent protein (mGFP; Figure 2f left panel).
To check the validity of the adjustments of the method made for bovine milk, the method was additionally validated at MRL using a single-day experimental setup.
Requirement of Cl− current in release of DNA was additionally validated by the cells in either Na+-free or Cl−-free medium, and subsequently DNA release was completely inhibited in Cl−-free medium.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com