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Removal of the affinity tag necessitates inclusion of an engineered protease cleavage site.
Apart from the moral status of the decisions made, the demand for transparent and democratically informed and supported policy that is pushed by such instrumental values has necessitated inclusion of citizen's perspectives (Wynne 2006).
Previous taxonomic revisions [ 53] that synonymized A. tenella and A. decemloba with A. obtusifolia necessitated inclusion of accessions attributed to the latter two species.
Only a randomised controlled trial embedded in a screening programme could give a more conclusive answer, although such a trial would probably necessitate inclusion of a prohibitive number of women.
Further refinement of the assay of Fang et al. (2012) would necessitate inclusion of more markers, which could be challenging due to the limited multiplexing capacity of a PCR.
Our model results show that health impacts (based on estimates of premature mortality following exposure to primary and secondary PM2.5) are likely significant enough to necessitate inclusion in a comprehensive evaluation of the benefits of measures to reduce congestion.
It necessitates a battle plan.
But it necessitates a rethinking of old ideas.
It necessitates simplified interfaces.
And it necessitates talking with your family.
It necessitates a multiplicity of minds.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com