The conserved residue Trp2, which is thought to insert itself into a hydrophobic pocket of another cadherin molecule (thereby providing the basis for cell-cell adhesion), switches conformation from exposed to intermittently buried upon removal of calcium ions.
We focus on FK1TMD, a short fragment composed of the first 36 N-terminal residues of fukutin, which is thought to insert into the membrane of the Golgi Apparatus.
The N-terminus of the ligand peptide are thought to insert into the orthosteric binding cavity, although alternative binding modes are also proposed (Beinborn, 2006).
In the presence of UDP (and cyclic-di-GMP), this loop moved 15 Å compared to the 'open' conformation and was inserted into the active site where it is thought to play a role in positioning the substrate donor [ 35].
In wild-type (WT) cells, this protein is thought to be first inserted into the ER, and then transported to the peroxisome via Pex19 (Elgersma et al., 1997; Hoepfner et al., 2005; Tam et al., 2005) (supported by data in Figure S7).
Exonization is thought to occur with such a retroelement inserted in the opposite orientation to transcription with the inverse "poly-A" tail of the retroelement forming a pseudo 3' splice acceptor site, along with de novo formation of a 5' splice donor site within the retroelement.
Insert it into the larger pipe and push it in, well past where the break is thought to be.
Both wild-type retroviruses and retroviral vectors were initially thought to insert randomly into the host genome, but it is now well established that the majority of them display a semi-random or a non-random integration profile possibly influencing the fate of transduced cells (Brady et al, 2009; Bushman, 2007; Cattoglio et al, 2007; Ciuffi, 2008; Mitchell et al, 2004; Wu et al, 2003).
Alu repeats were thought to be inserted very early in primate evolution, approximately 65 million years ago (mya).
Also some of the small imprinted genes that reside in an intron of other genes, such as Mcts2, Nap1l5, Inpp5f_v2, U2af1-rs1, and Nnat, are thought to be inserted into their present positions by retrotransposition.
